Basic workflow:
Nucleic Acid Extraction → Target Enrichment → Library Preparation →Clonal Amplification → Next-Generation Sequencing →Data Analysis & Interpretation
1. Library preparation system
Model: Qiacube Connect
- The QIAcube Connect performs fully automated processing of up to 12 samples
- The QIAcube Connect controls integrated components, including a centrifuge, heated shaker, pipetting system, UV LED and robotic gripper
- Sample preparation using the QIAcube Connect follows the same steps as the manual procedure (i.e., lyse, bind, wash and elute)
2. DNA, RNA, and protein quantification instrument (optional)
Model: QIAxpert
- Up to 16 samples in less than 2 minutes
- 2µl sample consumption
- Rapid analyse via touchscreen
3. DNA and RNA analysis instrument
Model: QIAxcel Advanced
- Analysis of 12 samples in as little as 3 minutes
- Unattended analysis of up to 96 samples
- Resolution down to 3–5 bp for fragments <500 bp
- Reliable detection with sensitivity down to 0.1 ng/µl
4. Clonal amplyfication instrument
Model: GeneRead QIAcube
- Capacity: up to 4 libraries pools per run
- Pipette system, robotic arm, centrifuge, shaker
5. Next-generation sequencing system
Model: GeneReader
Features:
- The GeneReader is designed to perform next-generation sequencing (NGS) applications by integrating highly parallel fluorescence-based sequencing chemistry with detection of the corresponding fluorescent signal
- The GeneReader workflow includes the following 6 processes: sequencing primer hybridization, flow cell preparation, reagents preparation, experiment set-up in QCI Analyze, flow cell loading and run start, and post-run maintenance wash
- Capacity: Up to 3 flow cells simultaneously
6. Data analysis and interpretation analysis software
Model: QCI PowerStation
- QCI Interpret you can annotate, assess and report NGS variants in the context of over 10 million relevant biomedical findings from the QIAGEN Knowledge Base