Basic workflow:

Nucleic Acid Extraction → Target Enrichment → Library Preparation →Clonal Amplification → Next-Generation Sequencing →Data Analysis & Interpretation

1. Library preparation system
Model: Qiacube Connect

  • The QIAcube Connect performs fully automated processing of up to 12 samples
  • The QIAcube Connect controls integrated components, including a centrifuge, heated shaker, pipetting system, UV LED and robotic gripper
  • Sample preparation using the QIAcube Connect follows the same steps as the manual procedure (i.e., lyse, bind, wash and elute)

2. DNA, RNA, and protein quantification instrument (optional)

Model: QIAxpert

  • Up to 16 samples in less than 2 minutes
  • 2µl sample consumption
  • Rapid analyse via touchscreen

3. DNA and RNA analysis instrument

Model: QIAxcel Advanced

  • Analysis of 12 samples in as little as 3 minutes
  • Unattended analysis of up to 96 samples
  • Resolution down to 3–5 bp for fragments <500 bp
  • Reliable detection with sensitivity down to 0.1 ng/µl

4. Clonal amplyfication instrument

Model: GeneRead QIAcube

  • Capacity: up to 4 libraries pools per run
  • Pipette system, robotic arm, centrifuge, shaker

5. Next-generation sequencing system

Model: GeneReader

Features:

  • The GeneReader is designed to perform next-generation sequencing (NGS) applications by integrating highly parallel fluorescence-based sequencing chemistry with detection of the corresponding fluorescent signal
  • The GeneReader workflow includes the following 6 processes: sequencing primer hybridization, flow cell preparation, reagents preparation, experiment set-up in QCI Analyze, flow cell loading and run start, and post-run maintenance wash
  • Capacity:  Up to 3 flow cells simultaneously

6. Data analysis and interpretation analysis software

Model: QCI PowerStation

  • QCI Interpret you can annotate, assess and report NGS variants in the context of over 10 million relevant biomedical findings from the QIAGEN Knowledge Base