The optics of the BD FACSCanto system consist of an excitation source with three lasers: blue (488-nm, air-cooled, 20-mW solid state), red (640-nm, 40-mW solid state), and violet (405-nm, 30-mW solid state).
Laser excitation optics illuminate cells in the sample, and collection optics direct light scatter and fluorescence signals through spectral filters to the detectors.
The excitation optics consist of multiple fixed-wavelength lasers, fiber optics up to the beam-shaping prisms, and achromatic focusing lenses that produce spatially separated beam spots in the flow cell.
The octagon contains 5 PMTs and detects light from the 488-nm blue laser. The trigons contain three PMTs each and detect light from the 640-nm red and the 405-nm violet lasers.
The BD MultitestTM 6-color TBNK kit is the first and only 6-color In Vitro Diagnostic application to provide a complete immune panel in a single tube, saving valuable time and resources for sample processing.